The best Spider-Man suit is from the 90's cartoon:
The original live-action Spider-Man trilogy has a great design too:
These also were the best versions of the character. My nephew was watching the cartoon a couple years ago and I hadn't watched it since I was a kid myself, but it holds up amazingly well, I was genuinely surprised. That theme song is still cool as hell too.
The original film trilogy is still the best live-action version as well. The second movie might be the best superhero film ever, actually (bar is pretty low imo). That train scene is still really cool and very well done.